The Trifecta was a trio of villains, recruited by Blaire Vherestorm for the purpose of pushing Mario and his allies to regain their powers. They were primarily after Mario, Luigi, Wario, Solid Snake, Ness, and Ryu. Merlin had told Wario to beware the Trifecta before he vanished, which means they formed sometime before the events of Operation: Blind Storm, likely between the events of :Act II Part 5" and "Act II Part 6" of the film.
- "You see, I needed you to get your powers back, so I told Blaire where he could find Liquid and Ken, and thus, the Trifecta was created. I knew that if you and your friends were threatened by a group of bad guys, that you would have no choice but to get your powers back. I was actually very lucky that Merlin gave Wario those hints."
- —The Darkness elaborating on the purpose of the Trifecta
Before battling Wario for the last time, the Darkness ordered Blaire to find Ken and Liquid so he could create the Trifecta. He helped the Darkness build the Beacon. After Mario and his best friends lost their powers in Operation: Blind Storm, the Darkness knew they would need to get their powers back. With their powers back, he would be able to take physical form.
Blaire was supposed to cease Trifecta activity the moment Mario and the gang got their powers back, but he decided to battle them instead. This resulted in his death at the hands of the Darkness for his disobedience. Liquid was killed in his fight with Snake while Ken was defeated in his fight with Ryu. Ryu let him go, not revealing that he himself was at his limit.
With Blaire and Liquid's deaths, the Trifecta was consequently disbanded.